Diffusion Potentials Across an Artificial Membrane

Membrane properties of Nafion epithelium (the base line for de-focus driven ocular growth)

SETUP AND PREPARATIONS

CHLORIDING THE ELECTRODE (Ag/Cl)





 SETUP OF THE SOLUTIONS AND USSING CHAMBER



USSSING CHAMBER



SOLUTION FILLED USSING CHAMBER WITH ELECTRODES



TOP VIEV OF RECORDING THE POTENTIAL



NAFION 324 IN USSING CHAMBER

Results so far... (subject to change)

Asymmetry potential _ 0.6 mV_

Nafion 115

Table 1 Diffusion Potential of Nafion 115

Test number		1	2	3	4	5	6	7	8
	Concentration of NaCl (Molarity)
Chamber 1		0.001	0.001	0.001	0.002	1.0	1.0	1.0	1.0
Chamber 2		0.001	0.01	0.1	1	0.002	0.01	0.1	1
Concentration Difference		1 X	10 X	100 X	500 X	500 X	100 X	10 X	1 X
Diffusion Potential reading(mV)	a.	0	-111.3		-125.0	114	85.4	2.5	0
	b.	0	-108.5		-123.5	113.6	86.5	2.4	0
	c.	0	-105.2		-123.2	113.3	66.4	2.3	0
Corrected Average reading*(mV)			-108.93		-124.5	113.03	78.83	1.8	0

* Average reading minus asymmetry potential


Nafion 324

Table 2 Diffusion Potential of Nafion 324

Test number		1	2	3	4	5	6	7	8
	Concentration of NaCl (Molarity)
Chamber 1		0.001	0.001	0.001	0.002	1.0	1.0	1.0	1.0
Chamber 2		0.001	0.01	0.1	1	0.002	0.01	0.1	1
Concentration Difference		1 X	10 X	100 X	500 X	500 X	100 X	10 X	1 X
Diffusion Potential reading (mV)	a.	0	-26.6		-104.4	115	75.4	2.4	0
	b.	0	-27.5		-104.	114.1	75.6	1.7	0
	c.	0	-28.6		-104.1	113.2	75.4	1.8	0
Corrected Average reading* (mV)			-28.17		-104.8	113.5	74.867	1.37	0

* Average reading minus asymmetry potential